![]() ![]() You must define the hypothesis or biological question that you want The LJI Flow Cytometry Core Facility is one of The LSRII uses Diva acquisition software and features Auto-Compensation and overall Fluorescence Tutorials. If anyone can provide updated links to the following, I’m happy to add them back to the list!Ĭhromocyte has their own list of software and other resources. COMPENSATION In modern flow cytometry, the way you start an experiment remains the same. Verity Software Tutorials (Modfit is at bottom of page) Also click on “Learn” from the homepage for more. CellQuest Pro Software Acquisition Tutorialįlowjo PDF and interactive tutorials. Detailed overview of the compensation theory underpinning our calculations. –BD’s online software tutorial for FACSDiva v6 on the LSRs (works in Explorer, I could not get it to work in Firefox.) Compensation was calculated ,checked and optimized with the single stain control data in the Matrix. To do this, you will select gates on positive and negative populations for each of these stain, and tell FlowJo to calculate the compensation matrix based on these stains. Simplify panel design today with FluoroFinder!īD Biosciences (Index of files here or go to and click on Support>Training and then click on “e-Learning”) : Flowjo v10.6.1 software was used to analysis the data. FlowJo computes the compensation matrix on control samples much the way you would manually set the compensation. 3) Select New Identity Matrix from the drop- down window. 2) Next, click the Create New Matrix icon (‘+’) in the bottom left corner of the matrix editor window. Many more features are available including selectors for Antigen Density, Viability Dyes, Fluorescent Proteins and a Dump Channel. 1) To begin creating a new compensation matrix, simply click on the compensation grid or ‘badge’ of a created matrix. Once designed, you can save, print, email, and share your panel with colleagues. Simply select your core facility cytometer, define your antibodies and dyes, and select products that work together on your cytometer. We will go into more detail regarding the important rules in Chapter 4, Controls in flow cytometry. The tool is both easy to use and powerful with over 100,000 products from 18 companies and growing. The definition of a compensation control is simple: for each fluorophore used in the experiment, a single-stained cell or bead sample must also be prepared. additional free programs reviewed and linked here:įluorofinder is a free online panel design resource for the flow cytometry community. Floreada a free web app for flow analysis. ![]()
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